DNA: 0.034 pg/diploid cell, 0.017 pg/ haploid cell
RNA: 1.9 pg /diploid cell, 1.2 pg / haploid cell
Protein: 8 pg/ diploid cell, 6 pg/ haploid cell
Range: Table - link pg/cell
https://bionumbers.hms.harvard.edu/bionumber.aspx?id=105079&ver=5
Reference: Sherman, getting started with yeast. Mehods Enzymol. 2002.
So, if Illuminia require 1 ng for sequencing, a single cell DNA needs to be amplified by:
1ng / 0.017 pg = 1000 pg / 0.017 pg ~ 1000* 1000 / 20 = 50, 000 X amplification.
50,1000 = 2**15, so 15 rounds of PCR amplification.
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