Wednesday, April 27, 2022

Seurat scRNA normalisation

 


The default normalisation in Seurat is pretty simple - it simply scales the counts by the total counts in each cell, multiplies by 10,000 and then log transforms.


https://www.bioinformatics.babraham.ac.uk/training/10XRNASeq/seurat_workflow.html#Normalisation,_Selection_and_Scaling




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