made 2 liter YPD plates

8pm-10pm.

10g YE
20g Peptone
20g Dextrose
20g agar
add 1000 ml ddH2O
autoclave for 30 minutes (70 minutes)

30 minutues, weight ingredients, 1 hour autoclave, pour plates (about 30 minutes)

Poured about 5 sleeves of plates.

YPD plate with glucose directly added

Make YPD plates with glucose directly added.

1. Dissolve 10g of BactoYeast extract in 500ml water
2. Dissolve 20g of BactoPeptone in the above solution
3. Dissolve 20g Dextrose in the above solution
4. Add 20g agar
5. Melt agar into solution in the microwave (skipped)
6. q.s. to 1000ml with water
7. Autoclave

See   http://www.thelabrat.com/protocols/YPDAgar.shtml

YPD plates