BIO125, Jan 21, 2014, MSH2, serial dilution with graphing paper and playdough

1.5 hours spent on going over reading assignments, the abstract of Gammie Genetic 2007. 

PubMed usage

MSH2

HNPCC

I used beads again to explain microsatellite and replication errors.

I spent about 40 minutes on sequence alignment, conservation to explain 'cognant' sites.

I use different models of cars to explain that 'wheels' and 'driver seat' are conserved, but the 'back seat' are not.

>1hour on serial dilution with graphing paper and playdough. 10X, 1X, 0.1X

Some students have trouble to understand 10X and 1X and how are connection to absolute concentrations.
C1V1 = C2V2
When applying the mass conservation formula, some students have trouble to decide which volume should the unknown.
 

Annoucement: read protocol 3.3, mathbench assignment 3.1 for quiz on Thursday.