Grow in SD-Trp-His-Ura media. Pick colonies from 20130114 plates. After four days, no growth. I suspect the old colonies on plat has lost their plasmids.
20140323: restreak plates of AGY124 and AGY125 from frozen stocks, Box 10, G4 and G5.
After two days, the colonies are healthy looking.
20140325. Grow cells in SD-His-Trp-Ura media. 30C shaker.
20140326. Cloudy culture. Take 100ul to 2ml water. Check with flow cytometer. Good signals for cells.
Use E03 for FSC, 952 for SSC.
References:
http://hongqinlab.blogspot.com/2015/01/bio125-strains-from-gammie-lab-media.html
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