-HIS to keep pMSH2
-TRP to keep pSH44 reporter plasmid
LB + amp because pRS413 has a beta-lactamase which offer resistance to beta-lactam antibiotics such as ampicillin and penicillin.
I added 5ul of 50 mg/mL ampicilin stock to 5ml LB.
Amplicilin, histidine stocks, LB and SC-His-Trp media are provided by Dr. Kioko.
20150113, 4pm, yeast liquid sample were at 30C shaker, and Ecoli strains at 37C shaker.
The yeast plate of AGY124 and 125 were at 30C incubator.
Plate AGY75 were left outside for histidine to dry.
20150114. 10am. Liquid growth are obvious for both yeast and Ecoli strains. Kioko froze the Ecoli in 20% glycerol as stocks.
AGY124 and AGY125 grew on SD-Trp-His-Ura plates. They form pink colonies when speaded dense population.
2015 Feb 12, I finally obtained SD-Trp-Ura plates, and spread AGY75 strains from the glass-tube cultures.
2015 Feb 16. AGY75 grows well on SD-Trp-Ura plate (Problem: some colonies became pink, but some remain white after a while. ).
2015 Feb 27 Fri, 9pm. Streaks AGY75 from Box10 G3 to SD-URA-TRP+HIS plate. Left at 30C incubator in Sci 246.
|Same plate, front view|