http://www.spelman.edu/docs/registrar/final-exam-schedule-spring-2015_v2.pdf?sfvrsn=2
Section 1: Final exam May 7, 2015 8-10am,
8:20am, asking student to spin down cells, add 500ul YPD. (many students did not resuspend the cells)
8:30am, incubation in YPD started.
8:40-9am. Went over pre-class assignments and in western blot assignment.
9am: Started site directed mutagensis primer design, cognat sites in multiple sequence alignment.
by 9:26am, finished three tutorial videos. Most students did not pay attention to the video. I then started the inclass assignment on G548C exercise.
10am, take tube from shaker, take 5ul from both HU+ and - on a single slide. (HU treated sample show some budding, maybe 50% from sample to sample).
10:12am, spin down, add 70% drop by drop to suspend the cells.
Cells often form clumps in 70% ethanol. So, Dr. Kioko votexed the tube and add 70% drop by drop.
I used a empty pipette tip box to store the cell and put them in the shaker. (This has no air, so it should be
Course evaluations were done at the end of section 1.
Section 2: final exam May 4, 2015 10:30 a.m. - 12:30 p.m
Students trickled in from a chemistry exam, complaining about having to finish 47 questions in 1 hour.
1:30, incubation in YPD started.
2:10-3pm, site directed mutagenesis. Codon bias, quarters by state, software sales, the more -> the better,
Most students did not pay attention to youtube video, but then complained about not knowing what to do. Some students try to use ApE to analyze protein sequences. Most students in section 2 worked alone and did not discuss with others. In contrast, many students in section 1 worked in groups.
3pm. take cells from YPD incubation. 5ul on slides. Some students have trouble to focus on the cells.
3:25pm. Ask students to do course evaluations.
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| Eppendorf tubes in a box at 30C shaker for incubation. |
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