MET15, rDNA on yeast Chromosome XII (12)

rDNA locus

Chromosome XII 458991..459097

http://www.yeastgenome.org/locus/S000120636/overview

According to Saka 2015 NAR, rDNA occupies 60% of chromosome XII. 

See:  Yeast rDNA Stability DataBase, YRSD at http://lafula-com.info/kobayashiken/geldata/index.php.

Met17/Met15 locus

Chromosome XII 732542..733876

http://www.yeastgenome.org/locus/S000004294/overview

streak met15+/-s strains

The strains mostly at the bottom of their vials due to freezer breakdown one time.
So, I let the vials thaw at RT from 4:21pm-4:30pm.
The vials were completely thawed after 10 minutes at room temperature.
I used suspended the cells with filtered tips, and put them a new MLA plate.


previous plate

I put strains on MLA plates. Some strains grew to tiny colonies in 1 day. Others took 4-5 days to form visible colonies.

DHE stain of HQ Met15+/- strains, YP2%D and YP0.5%D