rDNA locus
Chromosome XII 458991..459097
According to Saka 2015 NAR, rDNA occupies 60% of chromosome XII.
Met17/Met15 locus
http://www.yeastgenome.org/locus/S000004294/overview
Showing posts with label rDNA. Show all posts
Showing posts with label rDNA. Show all posts
Tuesday, May 10, 2016
Tuesday, July 9, 2013
DNA content, rDNA cluster size detection by flow cytometer
Marcus Nordberg used flow cytometer to detect genome size variation. He thought genome variation is primarily due to rDNA cluster size changes. In yeast, rDNA cluster size -> translational speed and accuracy OR extra-chromosomal rDNA circles OR genomic instability. rDNA and ribosomal function is related to lifespan. So, flow cytometer can be used to quickly phenotype rDNA sizes.
Alternatively, qPCR, short-reads mapping, and southern hybridization can be used. In this case, standardized rDNA clusters will be used to generate a standard curve. The rDNA cluster reference strains are available in David Bedwell's lab at UAB.
Alternatively, qPCR, short-reads mapping, and southern hybridization can be used. In this case, standardized rDNA clusters will be used to generate a standard curve. The rDNA cluster reference strains are available in David Bedwell's lab at UAB.
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