yeast double strand breaks, γ-H2AX and γ-H2B

 

Dynamics of yeast histone H2A and H2B phosphorylation in response to a double-strand break

https://www.nature.com/articles/nsmb.2737

In budding yeast, a single double-strand break (DSB) triggers extensive Tel1 (ATM)- and Mec1 (ATR)-dependent phosphorylation of histone H2A around the DSB, to form γ-H2AX

In Saccharomyces cerevisiae, histone H2A comprises the great majority of H2A isoforms and is phosphorylated on S129; we will refer to this modification also as γ-H2AX. Both in yeast and in mammals, γ-H2AX rapidly spreads on large chromatin domain (on more than a megabase in mammals and about 50 kb in yeast)

Both γ-H2AX and γ-H2B are strongly diminished over highly transcribed regions.