*** CRISPR/Cas9 in crevisiae

multigene knockout in yeast

[Mans15] Mans 2015 FEMS Yeast, CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae
Mans15 designed guide RNA (gRNA), a chimeric crRNA-tracrRNA, and provide a webtool, Yeastriction webtool, http://yeastriction.tnw.tudelft.nl/.

Yeastriction webtool. The tool is written in Javascript and based on the MEAN.io

stack (MongoDB, Express, AngularJS and Node.js). The source code is available at https://github.com/hillstub/Yeastriction. Genome and ORF sequences were downloaded from SGD

(http://www.yeastgenome.org) in GFF and FASTA file format, respectively. ORFs, including their 1 kb up- and downstream sequences, were extracted and imported into Yeastriction, with the aid of an in-house script