High-throughput measurement of single-cell growth rates using serial microfluidic mass sensor arrays
Nathan Cermak, et al, Nature Biotech, 2016
In Figure 5A, the semi-log growth of mass appears to be linear. So, the mass increase exponentially.
In Figure 5C, the growth rate increase is linear.
Figure 5 Measuring lag-phase yeast culture time-resolved growth dynamics at single-cell resolution. (a) Semilog plot of buoyant mass trajectories of yeast cells in rich medium. Data from SMR 9 were not used owing to very high noise. Particles between 5 and 20 pg are probably mostly single cells; particles with mass >20 pg are mostly clumps of cells. (b) Buoyant mass versus mass accumulation rate. Error bars indicate standard error of the slope estimated from each regression of buoyant mass versus time. (c) Mass accumulation rate per unit mass (interpretable as exponential growth rate) versus time. Error bars indicate standard error of the slope divided by the cell’s buoyant mass. Blue line shows locally weighted scatterplot smoothing (LOWESS) curve.
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