Wednesday, April 17, 2019

UTC travel reimbursment


Before travel, fill a T18 form

http://finance.tennessee.edu/wp-content/uploads/forms/T-18.pdf


After the travel

  1. Complete  and sign a T3 form  http://finance.tennessee.edu/wp-content/uploads/forms/T-3.doc
  2. Receipts of hotel, parking
  3. Meeting program,
  4. Your presentations
  5. A cover letter to explain the reasons for your travel, your activities, explain your actual travel arrangement.
  6. If mileage, print out round trip between home and meeting location


Tuesday, April 9, 2019

duplicate genes identification:


https://media.nature.com/original/nature-assets/ng/journal/v36/n6/extref/ng1355-S2.pdf

Identification of duplicate genes and singletons After database cleaning, we conducted an all-against-all FASTA3 self-search for the entire proteome of Drosophila melanogaster (http://www.ensembl.org/Drosophila_melanogaster/) and that of Saccharomyces cerevisiae (http://genome-www.stanford.edu/Saccharomyces/). A single copy gene (i.e., a singleton) was defined as a protein that did not hit any other proteins in the FASTA search with E = 0.1; this loose similarity search criterion was used to make sure that a singleton is indeed a singleton. Two genes were regarded as duplicate genes if they meet the following three criteria during FASTA all-against-all search (modified after Ref 4): (1) E = 10-10; (2) their similarity is ≥ I (I= 30% if L ≥ 150 a.a. and I = 0.01n + 4.8L -0.32(1 + exp(-L/1000)) if L <150 a.a., where n = 6 and L is the length of the alignable region); and (3) the length of the alignable region between the two sequences is >50% of the longer protein. Since we wanted to detect the differences in expression change between real duplicate genes and singletons, we