Wednesday, June 20, 2018

folder with large file size on applejack

Folders with large file size


applejack:MobileSync hqin$ pwd
/Users/hqin/Library/Application Support/Google
Google File stream temporary uploading file

/Users/hqin/Library/Application Support/MobileSync
iPad syn storage.

Monday, June 18, 2018

CRISPR in budding yeast

Cut
Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism which frequently causes insertions or deletions (indels) in the DNA. Indels often lead to frameshifts, creating loss of function alleles.
To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair (HDR) instead of NHEJ. In most experimental systems, HDR occurs at a much lower efficiency than NHEJ.
https://www.addgene.org/crispr/yeast/

There different types of CRIPSR/Cas kits: cut, based editing, nick, activate, and interference.

https://benchling.com/pub/ellis-crispr-tools

edX video files

Video files can be downloaded from EdX YouTube videos

Friday, May 25, 2018

NSF no-deadline calls




Update on No-Deadline

by dbiblogger
Following up on a previous post, “Upcoming Changes for Fiscal Year 2019 Award Submission,” new solicitations to replace previous solicitations in the DBI Research Resources Cluster will be made available after mid-year 2018.  Programs affected include:
  • Advances in Biological Informatics (ABI -NSF 15-582)
  • Collections in Support of Biological Research (CSBR - NSF 15-577)
  • Improvements in Facilities, Communications, and Equipment at Biological Field Stations and Marine Laboratories (FSML - NSF 16-506)
  • Instrument Development for Biological Research (IDBR - NSF 13-561)
For more information on the move to "no-deadline", please see the Dear Colleague Letter (NSF 18-011) and the Frequently Asked Questions.
Subscribe to the BLOG and stay tuned for updates!