yeast aging, somatic mutation, Dr. Dang

Dr. Dang Modulus aging model discussion

  DNA double strand break. Increased in old cells. Gamma H2A, phophlylation. A double strand breaker marker. Conserved in mammalian cells. It does mark single strand break. 

 https://www.nature.com/articles/leu20106#:~:text=Phosphorylation%20of%20the%20Ser%2D139,DNA%20damage%20initiation%20and%20resolution.

Yeast only has H2A (not H2AX). 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3889172/#:~:text=In%20Saccharomyces%20cerevisiae%2C%20histone%20H2A,about%2050%20kb%20in%20yeast).

  Mitosis crisis, short lived. Otherwise, long-lived. G1-death long0-lived. G2-death short lived. 

  SIR2-OE give third mode. 

  Rapamycin can be absolved by microfluidic device matrix, so it has been studied by microfluidics, according to Dang's conversation with N Hao

  TOR1Delta and SIR2-OE are additive in RLS, based on Dang’s own data.  Hong needs to check Kaberlein lab data sets.