__ take 100ul to 2ml water
__ point sonication, level 2, 4 times
Calibur, E03 FSC, 952 SSC, choose gate R1 for yeast cells.
template file: tempalte20150320-R1R2-v2
Thursday, March 26, 2015
*** Calibur, gating for wildtype and vector for bio125 MSH2 yeast strains
__ AGY 124 (vector control), AGY125(MSH2 control), grown in SD-His-Trp-Ura for 2 days
__ take 100ul to 2ml water
__ point sonication, level 2, 4 times
Calibur, E03 FSC, 952 SSC, choose gate R1 for yeast cells.
template file: tempalte20150320-R1R2-v2
__ take 100ul to 2ml water
__ point sonication, level 2, 4 times
Calibur, E03 FSC, 952 SSC, choose gate R1 for yeast cells.
template file: tempalte20150320-R1R2-v2
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