This site is to serve as my note-book and to effectively communicate with my students and collaborators. Every now and then, a blog may be of interest to other researchers or teachers. Views in this blog are my own. All rights of research results and findings on this blog are reserved. See also http://youtube.com/c/hongqin @hongqin
Monday, October 23, 2017
scientific format of floating point numbers in R
cellmap.org provide p-values in scientific format like 1.1e-01. R can read.table() this column as 'num'.
data for network changes during aging
Protein biogenesis machinery is a driver of replicative aging in yeast https://elifesciences.org/articles/08527 . This paper gives a 'directed graph' that can be used for controllability analysis.
CYCLOP: rapamycin data
Wednesday, October 18, 2017
Monday, October 16, 2017
NIH subcontract
Document needed.
1. Statement of Work
2. Budget
3. Budget justification
4. Biosketch
5. Facilities/equipment document
6. Letter of Support from Key Personnel
7. Institutional Letter of Support
yeast single cell protein levels http://cyclops.ccbr.utoronto.ca/
collection of yeast cells and localization pattern
http://cyclops.ccbr.utoronto.ca/
MYSQL is used.
http://cyclops.ccbr.utoronto.ca/
bout CYCLoPs
- The data stored in CYCLoPs correspond to studies described in Judice L.Y. Koh, Yolanda Chong, Brenda Andrews, Jason Moffat. CYCLoPs - A database of protein fluxes in Yeast Cell (Manuscript in preparation).
- 18 screens were conducted in the study, comprising of 2 chemical treatments and 1 genetic mutation, they include:
Screen Condition Time course Wild-type Screen Condition Time course Wild-type WT1 wild-type - - WT2 wild-type - - WT3 wild-type - - RAP60 rapamycin treatment 60 min WT3 RAP140 rapamycin treatment 140 min WT3 RAP220 rapamycin treatment 220 min WT3 RAP300 rapamycin treatment 300 min WT3 RAP380 rapamycin treatment 380 min WT3 RAP460 rapamycin treatment 460 min WT3 RAP540 rapamycin treatment 540 min WT3 RAP620 rapamycin treatment 620 min WT3 RAP700 rapamycin treatment 700 min WT3 HU80 hydroxyurea treatment 80 min WT3 HU120 hydroxyurea treatment 120 min WT3 HU160 hydroxyurea treatment 160 min WT3 rpd3del_1 rpd3 knockout - WT3 rpd3del_2 rpd3 knockout - WT3 rpd3del_3 rpd3 knockout - WT3 AF100 alpha factor treatment 100 min WT1 AF140 alpha factor treatment 140 min WT1 AF180 alpha factor treatment 180 min WT1
MYSQL is used.
fluorescent labels
http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins/Fusions/Red
http://www.clontech.com/US/Products/Fluorescent_Proteins_and_Reporters/Fluorescent_Proteins/Fucci_Cell_Cycle_Vectors?sitex=10020:22372:US&PEBCL1=PecQzqNVe9wzSLzBdE2uoNDO4A&PEBCL1_pses=ZGA8D8F214383771F0C0DB76A3075304A12F9A86A5827CFE3228A1B0DF9BA9D13CA93EB3593F96367AD0B93113BDB1E664E8DA5C56C31220DE
Friday, October 13, 2017
*** cpsc2100 software design and development, python
https://ocw.mit.edu/courses/electrical-engineering-and-computer-science/6-00-introduction-to-computer-science-and-programming-fall-2008/assignments/
https://courses.edx.org/courses/course-v1:MITx+6.00.1x+2T2017_2/course/
https://ocw.mit.edu/courses/electrical-engineering-and-computer-science/6-00-introduction-to-computer-science-and-programming-fall-2008/readings/
Using python for research
https://courses.edx.org/courses/course-v1:HarvardX+PH526x+1T2018/course/
python objects
https://courses.edx.org/courses/course-v1:HarvardX+PH526x+1T2018/courseware/6862667a32ef4801a65b558ca1b9361e/1dc918f84e7846fb90b2b05a9f4aa57f/3?activate_block_id=block-v1%3AHarvardX%2BPH526x%2B1T2018%2Btype%40vertical%2Bblock%40ad748d8eb5a14a0daf48a2be2f75473e
https://youtu.be/_RcVC9nSsGc
computer science for biology major books
https://www.amazon.com/Developing-Bioinformatics-Computer-Skills-Introduction/dp/1565926641/ref=sr_1_sc_2?ie=UTF8&qid=1507904075&sr=8-2-spell&keywords=bioinformtics+skills
https://www.amazon.com/Bioinformatics-Data-Skills-Reproducible-Research/dp/1449367372/ref=sr_1_1?ie=UTF8&qid=1507904147&sr=8-1&keywords=bioinformatics+skills
Tuesday, October 10, 2017
Saturday, October 7, 2017
Moxi flow, next generation flow cytometer
Moxi flow ~ $9000
moxiflow kits
https://www.orflo.com/product_p/mxf002.htm
flow cytometer cassettee, 2 test per cassette, $160 for 25 cassettee and 50 test. This means about $3.2 per test. For 100-day assay of 50 strains, it means $160*100 = $16K.
Moxi flow Go 2, $21K from VWR
https://us.vwr.com/store/supplier/id/0000012763/ORFLO+TECHNOLOGIES
next generation flow cytometer
https://www.ncbi.nlm.nih.gov/pubmed/28104919
https://www.nature.com/articles/nbt.1919.pdf
moxiflow kits
https://www.orflo.com/product_p/mxf002.htm
flow cytometer cassettee, 2 test per cassette, $160 for 25 cassettee and 50 test. This means about $3.2 per test. For 100-day assay of 50 strains, it means $160*100 = $16K.
Moxi flow Go 2, $21K from VWR
https://us.vwr.com/store/supplier/id/0000012763/ORFLO+TECHNOLOGIES
next generation flow cytometer
https://www.ncbi.nlm.nih.gov/pubmed/28104919
https://www.nature.com/articles/nbt.1919.pdf
Wednesday, October 4, 2017
Tuesday, October 3, 2017
Dell workstation
I don't know what your budget is, but we just purchased and configured a couple very nice Dell Precision 7810 Desktops. They each had 64GB RAM, 512GB SSD, and a 8 core Xeon chip, as well as a slightly upgraded NVIDIA GPU. These machines ran about $3500 each, a little on the high end, but quite nice. There were also a couple 24 inch monitors that we purchased with each desktop that ran about $350-$450 each; I don't remember the exact price. Basically, each desktop came with two monitors and we spend just under $4000 for each setup.
If that is too much, we could get the same machine with less exorbitant specs or simply purchase a couple Dell XPS desktops. I know that these are pretty decent but I'm not sure about the price right now. They'll be quite a bit cheaper. We have a pretty good relationship with Dell so we typically purchase from them.
So, I guess the first question is: what is your budget? That would be a good starting point for this conversation. Maybe, next time you're at the SimCenter, we can talk about it?
Sunday, October 1, 2017
Object-oriented design and patterns, 2nd edition
Object-Oriented Design and Patterns, 2nd Edition
http://bcs.wiley.com/he-bcs/Books?action=index&itemId=0471744875&bcsId=2562
Subscribe to:
Posts (Atom)

