I study computational and quantitative biology with a focus on network aging. This site is to serve as my note-book and to effectively communicate with my students and collaborators. Every now and then, a blog may be of interests to other researchers or teachers. Views in this blog are my own. All rights of research results and findings on this blog are reserved. See also http://youtube.com/c/hongqin
Monday, February 14, 2011
Bioscreen H2O2 titration
H2O2 titration in all Met15+/- strain.
Day 1, Feb 14, 2011 Monday, Grow all strains in 5ml YPD @30C
Day 2, Feb 15, 2011 Tuesday, Bioscreen titration
1:1000 Dilution in YPD,all strains to OD<0.03.
198ul yeast mix
2ul 100x H2O2 solun:30%20%10%5%2.5%1%0.5%0.25%0%
Final H2O2 solutions of 0.3%, 0.2%,0.1%,0.05%, 0.025%, 0.01%, 0.005%, 0.0025, 0% ,
For standard curves: use 3fold serial dilution of YPS128 and
M2-8. (200+400 dilutions)
For halo assay, add 5ul 30% and 15% H2O2 to the center of
each YPD plate. Measure inner and outer diameters.