Monday, February 25, 2013

Quantifying microbial cell growth, Hartman group and Xavier group


In Shah 2006 BMC Systems Biology,  the Hartman group used an ecology-based growth model to quantify yeast colony growth.

This model is an extension of the simple logistic function
where the numerator is replaced by the carrying capacity, and time is adjusted by TMR.

This paper provide the fitting results to  'wells' in a plate in S4 table. 

I did not find measurements for individual strains, perhaps because this is a method paper.


For comparison, the liquid culture growth curves are modeling by Ditmarsch & Xavier 2011 BMC Microbiology.  DX11 used Monon's equation based on nutrient concentration (N):


DX11 estimates the lag-phase between starting cell density X1 and X2 (X2>X1) by

Both approaches used the concept of carrying capacity.


Reference:
  •  Accurate, precise modeling of cell proliferation kinetics from time-lapse imaging and automated image analysis of agar yeast culture arrays, Najaf A Shah, Richard J Laws, Bradley Wardman, Lue Ping Zhao and John L Hartman IV, BMC Systems Biology, 2006. PubMed
  •  http://www.biomedcentral.com/1471-2180/11/140  Xavier group's liquid growth curve model.
2013 Oct 5,
The following 2012 NBT paper measured growth and aging in a modified BY4741 background. 
http://www.sysbiol.cam.ac.uk/Muellederetalnbt2442.pdf


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