5pm, yesterday, 1:10 dilution from stationary culture
today, 8am, OD=0.6 after 15 hours.
By 8:15am, 3 groups and 7 students were there. missing 3 groups.
Wait for students to trickle in. Play MSH2 project video. Ask them a question on alternative GT repeat design.
I used playdough beads to explain micro satellite repeats.
*After spin down, many students was not clear how to add 1mL water, what to transfer from the 15ml falcon tubes to 1.5ml eppendorf tube. This is a recurring problem.
*Some students were not clear which tubes contains the 1X dilution.
*some students did not suspend cells well before transferring.