Tuesday, April 7, 2015

bio125, hydroxyurea induced G1 arrest

X Email ppt to students

Section 1
bio125, hydroxyurea induced G1 arrest

check FOA plate
take pictures

http://en.wikipedia.org/wiki/Hydroxycarbamide

precision medicine youtube channel
whitehouse precicion medicine
https://www.youtube.com/watch?v=RIzbg8REzGw

http://www.pha.jhu.edu/~ghzheng/old/webct/note7_3.files/F13-22b.gif


MSH2 DNA mismatch-repair mechanism

at 10am, check +HU and -HU cells under micro scrope. The key difference is the missing of S-phased budded cell in +HU treatment. Yes, HU is working.


Section 2;

Same drill, and the experiment went more smooth.

FOA results in section 2 are mostly consistent with our expectaction.


Problems: 
Some student closed off the diagpham for microscope, some did not pull the switch for the vertical adaptor. Some did not know how to focus.




No comments:

Post a Comment