Wednesday, December 30, 2015

*** CRISPR/Cas9 in crevisiae

multigene knockout in yeast

[Mans15] Mans 2015 FEMS Yeast, CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae
Mans15 designed guide RNA (gRNA), a chimeric crRNA-tracrRNA, and provide a webtool, Yeastriction webtool,

Yeastriction webtool. The tool is written in Javascript and based on the
stack (MongoDB, Express, AngularJS and Node.js). The source code is available at and ORF sequences were downloaded from SGD
( in GFF and FASTA file format, respectively. ORFs, including their 1 kb up- and downstream sequences, were extracted and imported into Yeastriction, with the aid of an in-house script

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