advantages of the 561-nm (Yellow-Green) Laser for the flow cytometry.

a brief list of advantages of the 561-nm (Yellow-Green) Laser for the flow cytometry.

all the RFPs, GFP, CFSE and Fitc when really bright bleed into the PE channel horribly when using just a 488 laser. If you are using the 561 for PE there will be almost no spillover.

—  tdTomato detection (fluorescent protein 283% brighter than eGFP) 

—  MitoTracker Orange (accumulation is dependent upon membrane potential and the dye is well-retained after aldehyde fixation)

— CRISPR-Cas9, an emerging gene-editing (“genome surgery”) tool that frequently uses mCherry reporters. And yellow-green laser is required for optimal excitation of this fluorescent protein

— up to 3.7-fold increase in the stain index with antibodies conjugated with PE and PE based tandem dyes 

— greatly expands the choice of fluorochromes