single cell DNA reagent kits, library preparation

 
https://support.10xgenomics.com/single-cell-dna/library-prep/doc/user-guide-chromium-single-cell-dna-reagent-kits-user-guide

Solid Phase Reversible Immobilization (SPRI) beads are used to optimize the DNA size range for library preparation. see https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944320/

https://en.wikipedia.org/wiki/Single_cell_sequencing#Single-cell_genome_(DNA)_sequencing


This paper sheared all single-cell and bulk DNA, https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0105585 , "A Quantitative Comparison of Single-Cell Whole Genome Amplification Methods", de Bounrcy, 2014. This is an old paper though. This paper said that Illumina require 1 ng of DNA for sequencing. A single bacterial cell has 1 fg (femtogram) DNA, so, 1E6 application is needed. 


 
https://support.10xgenomics.com/single-cell-dna/library-prep/doc/user-guide-chromium-single-cell-dna-reagent-kits-user-guide
Solid Phase Reversible Immobilization (SPRI) beads are used to optimize the DNA size range for library preparation. see https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6944320/
https://en.wikipedia.org/wiki/Single_cell_sequencing#Single-cell_genome_(DNA)_sequencing