Tuesday, January 5, 2021

yeast cell DNA, RNA, protein

 DNA: 0.034 pg/diploid cell, 0.017 pg/ haploid cell

RNA:  1.9 pg /diploid cell, 1.2 pg / haploid cell

Protein: 8 pg/ diploid cell, 6 pg/ haploid cell

 Range: Table - link pg/cell

https://bionumbers.hms.harvard.edu/bionumber.aspx?id=105079&ver=5

Reference: Sherman, getting started with yeast. Mehods Enzymol. 2002. 

So, if Illuminia require 1 ng for sequencing, a single cell DNA needs to be amplified by:

  1ng / 0.017 pg = 1000 pg / 0.017 pg ~ 1000* 1000 / 20 = 50, 000 X amplification. 

50,1000 = 2**15, so 15 rounds of PCR amplification. 






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