Thursday, September 9, 2021

mummer, mummerplot ecs323gpu



mummer -maxmatch -n -l 100 ratg13.fasta prC31.fasta > ratg13-prc31.mumm

mummer -maxmatch -n -l 50 cov-fasta/ncbi-ref.fasta cov-fasta/ratg13.fasta > output/ncbiref-ratg13-09091457.mumm


mummerplot output/ncbiref-ratg13-09091457.mumm

mummerplot -x "[0,32000]" -y "[0,32000]" --png   output/ncbiref-ratg13-09091457.mumm



JC Question: Do mutation hotspots cover spike and Mpro regions? 

Based on NCBI NC045512: 
S gene is 21563 - 25384, which contain a large gap. 
3CL nsp5Ais 10055 - 10972.  The maxmatch results are: 
 ratg    wuhan  
 9227  9339 57
10487 10615 77
10661 10791 63
So,  the beginning and middle sections of 3CL are mutation hotspots too. 

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