Tuesday, July 9, 2013

DNA content, rDNA cluster size detection by flow cytometer

Marcus Nordberg used flow cytometer to detect genome size variation. He thought genome variation is primarily due to rDNA cluster size changes.  In yeast, rDNA cluster size -> translational speed and accuracy OR extra-chromosomal rDNA circles OR genomic instability.  rDNA and ribosomal function is related to lifespan. So, flow cytometer can be used to quickly phenotype rDNA sizes.

Alternatively, qPCR, short-reads mapping, and southern hybridization can be used. In this case, standardized rDNA clusters will be used to generate a standard curve. The rDNA cluster reference strains are available in  David Bedwell's lab at UAB






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