Tuesday, November 14, 2017
*** protein abundance in cells, yeast, paxdb
From W Dang, " a common control protein sample is mixed in the same quantity with all protein samples. Each mass spec peak is compared between the sample and the control, producing a ratio of sample/control for each protein". Does this "common control protein sample" refers to BY4741 young cells?
So, if we choose the protein abundance measurement from database, such as https://pax-db.org/species/
4932, we can use the ratios of "NR1 O/Y" to estimate the protein levels of "NR1 old cells" as:
NR1-old-cell-protein-levels = "NR1 O/Y" X BY4741-protein-levels
Likewise, we can use "CR1 O/Y" X BY4741-protein-levels = protein-levels-of-CR1-old cells ?
Do these make sense?