20150930Wed, Pick colonies for growth in 5ml YPD, 30C O/N in 10ml falcon tube. (Cell precipated at the bottom).
Made fresh YPD plates, 20% glucose.
20151001Thu. 5pm. Added 1ml of overnight grown culture into 10 ml of YPD in large glass tube. 30C shaker. A total of 4 tubes were prepared.
Friday, expand to 4 more large glass tubes
20151005 Monday
1pm: mix all tubes and do serial dilutions. I prepared 9 groups, but only 6 group actually formed.
2pm, bio233 lab. I did not do a demo for the class, and many mistakes happened.
1) many student put pipetman deep into glass culture tubes, and bunsen burners were not turned on.
2) many students did not know which pipetman to use for what volumes.
3) some students forgot to put cover skips on hemocytomers.
4) some students put glassbeads onto their gloved hands and then to plates.
5) many students did vortex their samples before put them on YPD plates.
3:45pm. Students put their number into master googleDoc sheet.
Items:
10 sets of pipettman. P1000, P200, P20. tips. YPD plates. Nutrient plates. Glass beads. Hemocytometer. Tally counters.
Reference:
http://hongqinlab.blogspot.com/2014/02/bio233-lab-serial-dilution-feb-26-2014.html
http://hongqinlab.blogspot.com/2014/10/bio233-20131006-serial-dilution-loh.html
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