Bio233, lab, serial dilution Feb 26, 2014

I had 10 minutes to set up this lab, because of another class in the same room.

Grow yeast SGU57 overnight. I then tried to create 5 OD value for standard curve analysis.
5, 4, 3, 2, 1 ml culture + 1,2,3,4,5 ml of fresh YPD.
OD600nm:

1	1	1.089
2		1.407
3	3	1.557
4	4	1.661
5		1.733

Serial dilutions were finished quickly. One student mistook 90ul as 900ul, and use P1000 to pick 100ul. One group labeled the yeast strain as 'bacteria'.

Student then worked on using hemocytometer in 5 groups. Initially, the challenge is what to look for, and which grid should be used. I tried Proscope on the screen but there were too many grid lines under the 10X and 40X. I had to switch to white board and sketch a picture to illustrate what to look for.  SGU57 yeast cells have many interconnected group like cell clumps. I asked students to count each individual cell, in order to show the different between hemocytometer and CFU.

The lab was video recorded to observe student engagement during the lab.

2014 March 19, added note:
Students can use googleDoc master sheet to input data, but have trouble to convert them to Excel for plot. I decided to convert the googleSheet to Excel and posted it on Moodle