Friday, February 1, 2019

*** desktop flow cytometer and cell counter survey

LUNA-FL . $9.7K 

Moxi Go 2, VWR $21K

Guava Millipore

CytoFlex $76K ~ $110K. 

advice from others:
"You might also look into the LUNA-FL or Countess FLII systems if the end use is as described, they are basically automated low-power epifluorescent scopes.  There are disposable and reusable slide formats. These are instruments much cheaper in comparison.  However, the Countess might not be able to assess yeast viability.

I have a Countess that we use for simple stuff, and we can get rough phenotypes for abundant surface markers.

I cannot recommend the Muse because it is my understanding that it’s completely kit based.  The Guava has been reliable in my experience, but I haven’t used one in a few years. "

SpectraMax® MiniMax™ 300 Imaging Cytometer, Molecular Devices, $45K 

"I strongly recommend trying your yeast samples on a demo Countess II/II FL instrument before purchasing one.  Yeast cells are generally very small and may be below the size cut-off for accurate counting and viability.  The instrument will see and count any cells larger than 4 um in diameter, but will only give correct viability for cells larger than 7 um.  If you open the histogram option on the Countess II results screen, you can see the diameter range the instrument sees for the sample. Unfortunately, we do not have example data for yeast.  "

Main fluophores: 
  Propidum Iodide (PI), dihydroethydium (DHE), and dihydrorhodamine (DHR)

My understanding of the basic configuration are (based on CALIBUR):
 blue laser 488nm + red laser 635nm (optional)
Detector channels: FSC, SSC, FL1 green 530nm, FL2 yellow 585, FL3 red 670nm, with an optional  FL4 661nm.

Vendors and models

BioRad S3e Cell Sorter


ACEA NovoCyte 1000 $40K ?
Novote Cyte model 2000, 3000 have more options. CytoFlex

Accuri C6PLUS  $49K

EmdMillipore Muse: $15K,nav#mch100102
Con: Closed system. Cannot be modified ?

Attune NxT Flow Cytomer

Stratedigm FLow Cytometer

Sales rep from BD on Gallios and CytoFlex
As per my voice message, I understand you are looking for a quote for 6 color Gallios Flow Cytometer, but we now have a new, much smaller flow cytometer with greater capability than the Gallios, and less expensive.   It is called the CytoFLEX Flow Cytometer.   The CytoFLEX is available in 21 different configurations from a 1 laser/4 color system to a 3 laser/13 color system in a small box (16in x 16in x 13in).   It is completely upgradable to any configuration at any time.   A 96 well plate loader option is available as are 561 nm and 375 nm laser options.   Independent testing has proven this instrument to be one of the most sensitive instruments in the market today.
 If the 561 or 375 nm lasers interest you, please let me know and I will forward additional information on our CytoFLEX “S” System.

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