Tuesday, July 19, 2016

AMNIS image flow cytometery

AMNIS, part of Millipore
FlowSight (smaller one, bench top), 20X 4-laser
20X (1 um per pixel)

ImageStream Mark II (full feature model), 20X, 40X, 60X, 405, 488, 561, 642 lasers
12 channels, 2 camera system
60X 0.33um per pixel

interactive GUI, each point can be clikced to show the cell image.


Aspect ratio, area of CD19-AF488 imagery
Valley mask

water quality test

R package for image-flow cytometer analysis?

IDEAS image analysis software, 85 features per channel.

signal to noise ratio? RD, a seperation ratio. Question on multiple-test correction. Rep suggested repeated experiments.

Machine learning methods to pick features or transformation of features?

Example on hematopoietic stem cells.

Quantify RBC heterogeneity.

3rd party software: ViSNE, cell profile, etc.



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