Tuesday, July 19, 2016
AMNIS image flow cytometery
AMNIS, part of Millipore
FlowSight (smaller one, bench top), 20X 4-laser
20X (1 um per pixel)
ImageStream Mark II (full feature model), 20X, 40X, 60X, 405, 488, 561, 642 lasers
12 channels, 2 camera system
60X 0.33um per pixel
interactive GUI, each point can be clikced to show the cell image.
Aspect ratio, area of CD19-AF488 imagery
water quality test
R package for image-flow cytometer analysis?
IDEAS image analysis software, 85 features per channel.
signal to noise ratio? RD, a seperation ratio. Question on multiple-test correction. Rep suggested repeated experiments.
Machine learning methods to pick features or transformation of features?
Example on hematopoietic stem cells.
Quantify RBC heterogeneity.
3rd party software: ViSNE, cell profile, etc.