Fluorescent substrates,
detection limits, 10^-21 moles
Sphigosine-FAM
Bodipy-PIP2,C4
Kinase/Protease substrates
Fast single-cell analyses
Microfluidic device, lyse cells by laser.
Sphigosine kinase: Single breast cancer cells, Red blood cells
Subpopulations of clinic samples can be detected. Heterogeneity in cancer cells were emphasized.
Fluorescent PIP2 reporter libraries.
FAM-PIP2
metabolism of PI3K
PIP2 --(PI3K) --> PIP3 ---(PTEN)-->PIP2 ... ...
Challenges: standardization between samples. Problems of cells lines.
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