*** human essential gene

NEST, sherley liu lab,
http://nest.dfci.harvard.edu/


CRISP screen paper from Feng Zhang lab:
http://www.ncbi.nlm.nih.gov/pubmed/24336571

Try to load the xlsx file into Rstudio. I waited for more than 50 minutes on Byte (4 G RAM), I then have to kill it. I then converted the xlsx to csv, and it worked in less than 1 minute!!!


> length(unique(tb$sgRNA.sequence))
[1] 64751
There are 64.7K CRISP shots.

Only 18.7K genes are tagged. So, the missing one contain essential genes. Some other criteria are needed. 
> length(unique(tb$Gene.name))
[1] 18736