bio125 HU treatment of yeast cells

Get the HU experiment start as quickly as possible.
During the 1.5 hour incubaion time,
check FOA plate, take pictures
go over group exercises. 


Section 3:
Projector is set at a wrong station. A technician came and figure it out. 





During the incubation, flip tube occasionally to make sure cells are suspended. 

HU made cells arrested at G1 phase with round shapes. 
 
Section 4:

Problems: Because we did not sonicate the cells, so often cells in G1 phases are often chained together.  It is easy for students to count big circle with tiny dots as S phases, and everthing else as unbudded.
 
References
http://hongqinlab.blogspot.com/2015/04/bio125-hydroxyurea-induced-g1-arrest.html
http://hongqinlab.blogspot.com/2015/03/hydroxyurea-treatment-of-yeast-cells.html
https://youtu.be/yxA1-MvwZfQ  2014 HU video