Thursday, April 17, 2014

Bio125, hallmarks of cancer, mutagenic primer design

Western blot video. 

I showed students of Kioko's video on transfer procedures. 

1hr on Hallmarkers of cancer

1 hr on mutagenic primer design exercises.
Students have trouble to count the AA positions in alignment. I used subtraction to locate the positions. Students have trouble with offsets of counting.

Dr. Kioko did a demo on second antibody stain and HRP staining. He showed a chem-illuniance in the tube, and students recognize the light from the glow sticks. Kioko told students that they are mostly generated using the same principle.

Corrected a mistake in online quiz. Most students tried, but some did not. 

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