yeast cell wall, membrane, nuclear envelope
Nuclear and cytosol
beta mecaptal ethanol
SDS PAGE gel
Protocol on board:
After adding blue ESB to the protein pellet, the TCA will turn ESB into yellow. Use Tris to adjust the color back to blue.
|Add DSB to pellet|
|Changed to yellow.|
|Adjusted to blue.|
MSH2 expression, cell cycle, log-phase
hMSH2 has no putative NLS signal, but MSH6 have 3.
co-import is supported.