QUBEHUB software for simulations

Including RStudio, XPPAUT, PPLane etc.

https://qubeshub.org/resources/software

PLAS power law analysis and simulation

Voit book, software support for ODE analysis

http://enzymology.fc.ul.pt/software/plas/

Atlanta QBIO book orders

Books to order:

A first course in systems biology, By Eberhard Voit

ISBN: 9780815344674, Garland

$135

Physical models of living systems,  by Philip Nelson

ISBN-10: 1-4641-4029-4; ISBN-13: 978-1-4641-4029-7;

$150

Mathematics for the life sciences,  by Erin N. Bodine, Suzanne Lenhart, Louis Gross

ISBN-13: 9780691150727, Publisher: Princeton University Press

$70

A Biologist's Guide to Mathematical Modeling in Ecology and Evolution, by Sarah P. Otto Troy Day , ISBN: 781400840915,  Publisher: Princeton University Press

$80

Mathematical Modeling in Systems Biology: An Introduction, by Brian P Ingalls,

Publisher: The MIT Press; 1 edition (July 5, 2013), ISBN-10: 0262018888, ISBN-13: 978-0262018883

$55

Data Wise, Revised and Expanded Edition: A Step-by-Step Guide to Using Assessment Results to Improve Teaching and Learning

http://www.amazon.com/Data-Revised-Expanded-Step---Step/dp/161250521X/ref=sr_1_4?ie=UTF8&qid=1461701152&sr=8-4&keywords=education+assessment

Discipline-Based Education Research: A Guide for Scientists Paperback – July 16, 2015

by Stephanie J. Slater  (Author), Timothy F. Slater  (Author), Inge Heyer  (Author), Janelle M. Bailey (Author)

Be the first to review this item

http://www.amazon.com/Discipline-Based-Education-Research-Guide-Scientists/dp/1515024563/ref=sr_1_fkmr0_1?ie=UTF8&qid=1461701711&sr=8-1-fkmr0&keywords=Discipline-based+education+research+%E2%80%93+A+guide+for+scientists%2C+2nd+Edition.


References:
http://www.lifescied.org/site/misc/ifora.xhtml

-Schneider B, Carnoy M, Kilpatrick J, Schmidt WH, & Shavelson R. (2007). Estimating causal effects: Using experimental and observational designs. American Educational Research Association: Washington DC.

-Shavelson RJ, & Towne L, Eds. (2002) Scientific research in education. National Academies Press: Washington DC.

-Singer SR, Nielsen NR, & Schweingruber HA, Eds. (2012). Discipline-based education research: Understanding and improving learning in undergraduate science and engineering. National Academies Press: Washington DC.  

-Slater SJ, Slater TF, Heyer I, & Bailey JM. (2015). Discipline-based education research – A guide for scientists, 2nd Edition. Pono Publishing: Hilo, Hawai’i.

-Weimer, M. (2006). Enhancing scholarly work on teaching and learning. Jossey-Bass: San Francisco.

(to read) Kaya, Ma, sanger strain lifespan study

 Defining Molecular Basis for Longevity Traits in Natural Yeast Isolates

Alaattin Kaya1,#, Siming Ma1,#, Brian Wasko2, Mitchell Lee2, Matt Kaeberlein2, and Vadim N. Gladyshev1,*

1Division of Genetics, Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, 02115, USA

RLS were provided

todo: download data, and Sanger resource data

Growth rates were determined using a Bioscreen C MBR machine by analysis of optical density in the OD420-580 range as previously described in combination with the YODA Software package18. The data on transcripts, peptides (proteins), metabolites and morphology were downloaded from Yeast Resource Center http://www.yeastrc.org/g2p/download.do. Values corresponding to the 22 strains were extracted; metabolite data were not available for 378604X. Metabolites with missing values in more than one strain (other than 378604X) were discarded; the remaining missing values (6 out of 107 metabolites) were imputed based on 10 nearest neighbors, using “knnImputation” function of R package “DMwR”. For comparison across the phenotypic data, the values were standardized across the strain by setting mean = 0 and standard deviation = 1. In addition, for genes represented by multiple peptides, we calculated the mean standardized values to perform the regression.

(to read) Yang 2015 PNAS yeast GFP screen, asymmetric partition

GFP library screen, Cy5 labeling of cell wall, daughters have few cy5 because their walls are newly synthesized.

Most proteins are symmetrically distributed.

All aging factors are 'non-essential'.  This means that their 'interactions' to essential genes should be of importance in my network aging model.

yeast DNA repair genes

Gene Ontology Term: DNA repair

---

GO ID

GO:0006281

Aspect

Biological Process

http://www.yeastgenome.org/go/GO:0006281/overview

SIR2
protein  NP_010242.1
http://www.ncbi.nlm.nih.gov/protein/6320163?report=fasta

https://www.ncbi.nlm.nih.gov/nucleotide/296143322?report=genbank&log$=nucltop&blast_rank=1&RID=HJD968YR01R

RFA1

bio125 20160420 flow cytometer data analysis in R,

Section 3
video recording

9:10-10:10am, Flow cytometer data analysis in R using my own laptop. Helped some students with RStudio package installations.

Problems: In Windows 10, Rstudio has to be run as administrator to install packages.

10:15am, course evaluation
10:25am post-computing survey
10:35am lab 11.1_group

section 4

1-2pm. R Rstudio on flow data anlysis
Problems: R3.2.3. installation.  a/s/n warning.   Jumping lines. Setting working direcories.

2:11pm. Post survey

by 3pm.  review bioinfor_1

BIO125, 0420 Wed flowcytometer data analysis

https://github.com/hongqin/SBIO125/tree/master/20150415PI

toread, yeast RLS screen paper

http://www.cell.com/cell-metabolism/abstract/S1550-4131(15)00465-9?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1550413115004659%3Fshowall%3Dtrue

toread, Yeast longevity promoted by reversing aging-associated decline in heavy isotope content

Yeast longevity promoted by reversing aging-associated decline in heavy isotope content

• Xiyan Li
•  & Michael P Snyder
• • http://www.nature.com.proxy.library.emory.edu/articles/npjamd20164?WT.mc_id=SPG_AMD_1602_apac1
  • • 
      

stochastic modeling of histone modification in yeast

mating phenotype maintenance?

CR SIR2 histone modification modeling

Reference:
J Xing's similar work on histone modification

CR, rapamycin effect on LOH in CLS and H2O2 treatment, NIH R15?

CR, rapamycin effect on LOH in CLS and H2O2 treatment

Human essential genes?

Mining  shalem 14 data set

References: NEST in in shirley liu's lab.

Project Achilles
http://www.broadinstitute.org/achilles

*** human essential gene

NEST, sherley liu lab,
http://nest.dfci.harvard.edu/


CRISP screen paper from Feng Zhang lab:
http://www.ncbi.nlm.nih.gov/pubmed/24336571

Try to load the xlsx file into Rstudio. I waited for more than 50 minutes on Byte (4 G RAM), I then have to kill it. I then converted the xlsx to csv, and it worked in less than 1 minute!!!


> length(unique(tb$sgRNA.sequence))
[1] 64751
There are 64.7K CRISP shots.

Only 18.7K genes are tagged. So, the missing one contain essential genes. Some other criteria are needed. 
> length(unique(tb$Gene.name))
[1] 18736

.gitignore file

.gitignore


https://git-scm.com/docs/gitignore

Resazurin Cell Viability assay

http://www.cellsignal.com/products/cellular-assay-kits/resazurin-cell-viability-kit/11884

Elastic net regression

Elastic net regression

http://www.r-bloggers.com/kickin-it-with-elastic-net-regression/

***, compound profiling, initial thoughts

These compounds are 'newly designed and synthesized' and their targets are needed to be identified and verified.

PCA, then, Euclean distance,  MCL
PCA, then K-means

For comparison: Elastic net

Score criteria: jaccard index with original annotation.

https://en.wikipedia.org/wiki/Jaccard_index

GIS commons

http://giscommons.org/earth-and-map-preprocessing/

bio125 cancer and ucsc genome broswer

Section 3:
2015 bio125 assignments are based on GRC37/hg19 assembled in 2009.
4 exercises in class
10:30am, class is over.

bio125 HU treatment of yeast cells

Get the HU experiment start as quickly as possible.
During the 1.5 hour incubaion time,
check FOA plate, take pictures
go over group exercises. 


Section 3:
Projector is set at a wrong station. A technician came and figure it out. 





During the incubation, flip tube occasionally to make sure cells are suspended. 

HU made cells arrested at G1 phase with round shapes. 
 
Section 4:

Problems: Because we did not sonicate the cells, so often cells in G1 phases are often chained together.  It is easy for students to count big circle with tiny dots as S phases, and everthing else as unbudded.
 
References
http://hongqinlab.blogspot.com/2015/04/bio125-hydroxyurea-induced-g1-arrest.html
http://hongqinlab.blogspot.com/2015/03/hydroxyurea-treatment-of-yeast-cells.html
https://youtu.be/yxA1-MvwZfQ  2014 HU video

bio325 guest lecture, yeast oxidative stress analysis

Try to lead students work on Rmd file for yeast gene expression data analysis

It turns out that the bioconductor packages are not supported by the recent R3.2.4. I had to lead students to use R3.2.3.

Among the students, there are 7 Apple laptops and 2 windows laptops.

I made a mistake when going through probes on Affymetrix Yeast 2.0 chip. It contains 2 MSH2 genes, on for S cerevisiae and one for S pombe.

toread http://bromberglab.org/?q=publications

http://bromberglab.org/?q=publications

23andMe in courses

23andMe in courses

Udacity course
https://www.udacity.com/course/viewer#!/c-bio110/l-301595193/e-302316765/m-302316766

MSH2, DNA mismatch repair, and sporadic colon cancer

BIO125 used MSH2
A Howard U and Johns Hopkins study on sporadic colon cancer and DNA mismatch repair
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713502/


Primer design to amplify your DNA signature, identify RE to distinguish the polymorphic sites.

open human, data source

https://www.openhumans.org/

Personal Genome Project,

Personal Genome Project

https://my.pgp-hms.org/public_genetic_data?data_type=23andMe

Why the data vary from 5M to 200M?

spelman biology curricula

Competence-based core curriculum. reading, writing, talking and doing science.

SAWOK, science as a way of knowing
http://www.spelman.edu/docs/academics-biology/bio_sawok-classes_spring2012.pdf?sfvrsn=0

Morehouse
http://www.morehouse.edu/academics/bio/pdf/Biology-Dept-Handbook-082514.pdf


Gene and genealogy resources
http://dna-explained.com/category/education/

23andme Data Format, technology

The file 23andme provides has four columns: 
rs ID, chromosome, position, and genotype


Beadchip for genotyping
https://www.23andme.com/more/genotyping/

Bioconductor 23andme

http://www.vincebuffalo.com/blog/2012/03/12/using-bioconductor-to-analyze-your-23andme-data.html

bio233 order list fall 2016

Wish list for bio233 orders

** marker pens
** 10 tally counters
** 95% enthanol large order for 70% enthanol bottle
**latex-free gloves
** lens cleaners

swim noodles
colored bacteria for edutainment

cover slips, fix

slides for gram stains: spirilum +/-, bacillus +/-, cocci +/-

x brightline hemacytometer chambers

scissors
scotch tapes
play doughs

nutrient plate agars

small bottles for ethanol. Idodine. Crystal violet.

Yeast selective media, drop-out media

bibulous papers
filter papers

BPB pH color indicator for fermentation lab. 

BD Calibrite beads

15ml falcon tubes

2016 research day preparation

Research posters:
Maya Jones
Faith Lyons
Maya Bryant
Jessica Corley


BIO125 course posters
Gaina-Yvvan Pierre, Elsie, Unique Hayes
Jaliyah Peterson, Lady Nwdike


Oral presentations:
 Kierra Parker
 Imani-Michelle White

bio125, 20160401Fri FOA

Materials: FOA plates, cells, serial water, Eppendorf tubes.

Section 3:
Ask the purpose of URA3 and FOA,

9:20, draw a diagram on the board. 

Go over previous student mistakes, not mix cells well, label the cover, adding cells to the cover. 

9:30, students started the lab. Students should have 9 tubes, and can be balanced triangularly in centrifuge. 

9:47. Students cannot see the pellet for OD=0.01. I remind them to orient their tubes the same way, so they can know where the pellet positions, even they cannot see the pellet.
One group only labeled OD on the tube but not strain names, and are confused after centrifuging
by 10:30, four groups are still working on the lab, because they have to redo the dilutions.
Forgot to ask students to label their section, and group on plates.

Section 4:

Emphasize that tubes during spin should be arranged 

One group was not sure that tips need to be changed each time for a different dilution.

Reference:
http://hongqinlab.blogspot.com/2015/04/bio125-foa.html
http://hongqinlab.blogspot.com/2014/03/bio125-foa-assay-of-msh2-mutants.html