Thursday, March 27, 2014

bio125, FOA assay of MSH2 mutants

Spin down 5 ml of culture (SD+LEU), wash with 1ml water, resuspend in 100 ul water, do 10X, 100X dilutions, then spot 90ul on FOA plate.

The FOA plate is -histidine - tryptophan - leucine -theorine + FOA.

Several groups did not draw the 3x3 square correctly. We found that the marking can be wiped off by ethanol and redrawn.

Three out of the five groups did not pipetted the small volumes correctly or did not mix the cells well before loading to FOA plates.

Two groups did not label the FOA plates and had to label the cover after sample are spotted.

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