=> temperature sensitive genetic interactions
4.4x more than the 2010 version.
negative essential interactions are functionally informative (are locally important).
In other words, possitive genetic interactions are long-range.
=> sirtuins substrates, David Paul Toczyski
nitotinamide treatment, quantitatvie mass-spec
52 substrates of sirtuins, many are TFs, growth and ribosome biogenesis
=> Ohnuki, Ohya, phenotyping in haplosufficiency in essential genes
ribosomal complex
chaperonin CCT complex
positive correlated GO terms:
netative correlated GO terms: proteosome <--> tRNA synthesis
=> disordered proteins, Daniel Jarosz
20 yeast proteins with prion domain (N/Q rich)
screens show many more, suggest more non-canonical prion-domains
GAR+ loves longer than gar-
bacteria elicit [GAR+] via a small molecule (unknown)
GAR+ coexist with bacteria
=> Hofmann
Ure2, Cyc8, Sfp1, Mot3 --> FLO11, HXT2
predicted prion TFs
FLO11 bistable
Holmes 2013 Cell
fermentable carbon ~ nonfermentable carbon
azide blocks electron transfer chain
FLO11 is heavily glycosylated, a glucose sink
Iodine staining of colony (glycogen)
50 nM R3a-5a small molecule inhibitor of manno-transferase?
Dan G: why cheater? Just two mode of development states? (It is the same genome.)
=> Ben Tu's lab, mitochondria biogenesis
yeast metabolic cycles
glucose depletion and ethenao+glycerol induce mitochondria biogenesis
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