bio233, flow cytometer lab on DHE-labelled yeast cells.

 The class spent 2 hours on DHE staining. I could save the time by giving assignment on the protocol itself.

When I let students to re-streak their plates, some groups stopped working on their DHE staining procedure. 

At 4pm, I started the Cellquest but no signal can be read from the Calibur. This is the 3rd time that this machine malfunctioned. Really bad timing.