bio233 guest lecture
CAPP-seq
CT, biopsy are common method for tumor diagnosis.
How did ctDNA comes from tumor?
There arre much cell-free DNA in human circulation, typically 5ng/ml of plasma in healthy adults, primarily from hemopoetic cells. Cell-free DNA often have half-live are 0.5 ~ 2 hours.
Hybrid selection (NimbeGen), target enrichment.
10,000X sequencing is required?
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