=>dilute plasmid to 5 ng/uL
9:25am, went over recipe
add large volume of water first. For 200uL pipette, round up the volume to the nearest integers.
Take away concentrated plasmids.
=>PCR master mix.
Go over PCR master mix recipe
9:38am PCR beads are given to students
Problems: One group added master mix into diluted plasmids.
=> try respondus lockdown broswer
=> 30 minutes on PCR paper exercise.
1:30pm, most group submitted wrong plasmid dilution recipe.
by 2pm, at least 3 groups have not started to dilute their pasmid DNA.
by 2:20pm, some groups finished their PCR reactions.
ask a group to finis paper pcr on boards.
homework common problems:
Wrong: HNPCC has no symptoms, a google trap.