Tuesday, February 18, 2014

bio125, PCR reaction

things covered: pcr principle, primer design. dilution of plasmid to 5ng/ul. GC content, annealing temperature and DNA melting temperature.

students asked for MSH2 alignments. I took the opportunity and give an overview of the MSH2 project.


pcr ready to go beads can fly out of the tube if they are electrostatic.








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