This site is to serve as my note-book and to effectively communicate with my students and collaborators. Every now and then, a blog may be of interest to other researchers or teachers. Views in this blog are my own. All rights of research results and findings on this blog are reserved. See also http://youtube.com/c/hongqin
@hongqin
Tuesday, February 18, 2014
bio125, PCR reaction
things covered: pcr principle, primer design. dilution of plasmid to 5ng/ul. GC content, annealing temperature and DNA melting temperature.
students asked for MSH2 alignments. I took the opportunity and give an overview of the MSH2 project.
pcr ready to go beads can fly out of the tube if they are electrostatic.
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