8-8:45am Go over PCR protocols, using R code for dilution. Make master screen cast.
Group 2 worked out the master mix on the board.
Each rxn: 2 uL of primer mixture and 23uL water for each reaction + 1 bead
2 uL 5n/uL template
by 9:30 Setup PCR. (Warning: Glove and PCR bead can attract with electrostatic pull)
First group finished setting up PCR, but some groups still work on the template dilutions.
I asked the leading group to follow youtube video and work on primer analysis.
10-10:30am
Screen cast:
PCR primers on pMSH2, followed by restriction enzymes
https://www.youtube.com/watch?v=aE8UUGJYzHw&feature=youtu.be
Problem: some students choose 'circuar' for PCR framgment.
ApE PCR primer analysis and followed by restriction enzyme, gel simulation
PCR primers on pMSH2, followed by restriction enzymes
https://www.youtube.com/watch?v=aE8UUGJYzHw&feature=youtu.be
Problem: some students choose 'circuar' for PCR framgment.
ApE PCR primer analysis and followed by restriction enzyme, gel simulation
Go over lab report: RE lab report will be due, video and R code
Section2:
Only five students were in by 1:10pm.
1:15pm start class.
2:10pm students started setting up reactions.
by 3pm. video to use provided RE to analyze PCR fragments of WT and msh2 mutants.
Unused and Moved to next week
PCR_inclass_data analysis
BLAST
Past exam questions on gel pictures.
todl Design site-directed mutagenesis primers
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