1hour, went through protocol for XbaI and Apa1 cutting.
Use ApE to do XbaI and ApaI mapping. Dr. Kioko ordered FastDigest enzyme and buffer. Both enzyme work at 37C. The 37C incubation did not finish because the heat block somehow could not reach 37C.
Explained why 6bp cutters have fewer sites than 4bp cutters. Most students use plus instead of multiplication to do the calculation. I used 3 colored play dough again to enumerate the 9 possibility for 2 positions. I initially tried 2 colored play dough, but ran into the problem of 2+2 and 2x2 yield the same result.
Kioko mixed XbaI and ApaI. Student took 1ul of the mixture.
No comments:
Post a Comment