Lab started around 2:05pm.
Two students explained procedures on board. I did demo on preparing smear and gram stain.
Problems and issues that need to be addressed in the future:
Some slides are oily, and cells could not be spread on them.
Some students are not sure how much water they would put on slides, or how to spread the water thinly.
Some students will do Gram stain in their own space instead of the sink.
Some over-decolorized the stain with 95% EtOH.
Some did not sufficiently decolorize the stain or add too much stain. Some crytal violet and safranin contain too much debris. New bottles of reagent should be bought when funds are available.
Several students did know how to use microscope properly.
Most students finished the lab within 40 minutes. Three students worked on their own and spent about 20 more minutes. Overall, the lab finished at 4:30pm.
Students could use their iPhones and took picture from the eye-pieces.
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| Gram-positive Bacillus megaterium |
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