Tuesday, February 25, 2014

bio125, RE of pcr fragment to verify mutation in msh2, Feb 25, 2014, research day abstract.

Each group choose an RE to cut the PCR fragment of pmsh2, pMSH2, vector. We started with reading protocol and write the procedure.  This took about 45 minutes.

Set up reaction, takes about 30 minutes. One student mistook ladder as load dye. Some are not clear what what sample goes to what tubes.

During the digestion, we worked on abstract. "What's our topic" is a common question.  Most groups are behind on their lab report.

During the gel loading, some students loaded sample to the running buffer, and some punctured into the agarose gel.  The gel were run under 120 Volts.  Dr. Kioko took the pictures around 5pm.

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