bio125 Tue, Feb 10, 2015, PCR principle

Before class: 
1) color-print PCR paper exercises
2) MCAT PCR questions

Section1:
8-9:30pm, in class ApE and identify mutation. Identify mutation in each group.

30 minutes PCR paper exercise: ask student to write PCR procedure first.

20 minutes, Analyze gel pictures. Example of how to label a gel picture.

last minutes, asked students to go around and recap what have learned.

Section2:

1-2pm, went over geneX exercise

2-2:44, identify mutant in each group. 

2:45-3:20 PCR paper exericse. 

Lab instructor observed that some students try to put both forward and backward primers on the same template. 

Did not have time for gel analysis for section 2.

Students came out of a chemistry exam and claim an open book component will be due by 6pm. In the bio125 class, many students were busy working on their chemistry exam. 

Review RE exercises

Show a youtube video. I can ask one student group work on the board with tapes and markers.
https://www.youtube.com/watch?v=_YgXcJ4n-kQ
Materials: Razors, scotch tapes, color markers

PCR MCAT questions

Past exam questions on gel pictures.

Thu:
Design site-directed mutagenesis primers

Reference:
http://hongqinlab.blogspot.com/2014/02/bio125-re-of-pcr-fragment-to-verify.html