bio125, Feb 17 Tue 2015, PCR exercise (lab postponed due to icy weather)

Section 1: class canceled. 2014 midterm exam in class time.

Section 2:
1:10pm, class started.

by 1:50pm, go over PCR case study problem on pin3. The last question has not enough information. One of the choice is also wrong, because heterozygous should give 2 bands. 

1:50pm - 2:50 pm. 

In class mutation identification exercise. Some students did not know which tools to use. 

ApE primer analysis

2:50-3:20pm. MSH2 project overview. 

3:20-3:30 MCAT PCR exercise. 

Asked a student to summarize the class activities.

Presentation ended in group 1.

Not used. 

Cognant sites between human and yeast MSH2
Design mutagenic primer

Design mutagenic primers
https://www.youtube.com/watch?v=Qy_Z2H_KAwo

http://hongqinlab.blogspot.com/2014/04/design-mutagenic-primer-to-engineer.html

Learning objectives: Cognant site between human and yeast, preferred codons, primer design

Review bio125 midterm exam from previous years

Postponed RE cut of PCR fragments
Ref:
http://hongqinlab.blogspot.com/2014/02/bio125-re-of-pcr-fragment-to-verify.html